Flasks containing cells in culture in an incubator

Lacucel logo and registry number of the Madrid's Community laboratory network

The Cell Culture Laboratory is part of the Madrid's Community Laboratory Network and has the following technical capabilities:

  • Generation of human and mouse mesenchymal stem cells (MSCs) of different origin: bone marrow, adipose tissue, peripheral blood.
  • Expansion, differentiation and characterization of MSCs, both under conventional and genetically modified conditions. Transduction of MSCs with suicide genes and marker genes.
  • Generation, expansion and characterization of human MSCs from patients with Fanconi Anemia.
  • Optimization of human MSCs culture conditions from patients with Fanconi Anemia.
  • Maintenance of primary cultures and cell lines.
  • Obtaining and purifying hematopoietic progenitors from mouse models with defects in the hematopoietic system.
  • Obtaining, purifying and expanding hematopoietic progenitors from samples from patients with Fanconi Anemia and Pyruvate Kinase deficiency.
  • Maintenance of production lines for retroviral supernatants.
  • Generation of retroviral supernatants for the correction of hematopoietic progenitors with defects in the hematopoietic system.
  • Human embryonic cell culture.
  • Establishment of protocols for the differentiation of human embryonic cells into cells of the immune system: T cells, NK cells.
  • Obtaining iPS (cells with induced pluripotency) from human and mouse adult cells reprogrammed with polycistronic vectors.
  • Establishment of iPS differentiation protocols towards cells of the hematopoietic system.
  • Generation of genetically corrected iPS from samples of patients with genetic diseases that affect the hematopoietic system.
  • Obtaining disease-free hematopoietic progenitors from genetically corrected iPS, capable of reconstituting the hematopoietic system.

Lacucel, within its facilities has the following associated laboratories:


Associated laboratories logo: Clinistem and Laprovec


For more information, you can consult our website.